· PolyacrryGel ga-eji akwụkwọ polyMamide, polymerization amalite ihe, ihe na-eweta ihe, na ikike nke nnu na ihe nri ngwakọta ọnụ.
· Acrrylamidena bis (n, methylene okpukpu abụọ acrrylamide) bụ monomer ụdị gel matriks.
Starmerizate na-amalite usoro polymerization. Ntoputa nke gluu chọrọ 10% ammonium paseeji kwadebere na mmiri. Imirikiti ozi ahụ gosipụtara mkpa maka iji rụọ ọrụ. Agbanyeghị, enwere ike itinye ngwọta 10% 10 ℃ ọtụtụ izu na-enweghị ọrụ dị ukwuu. Ruo 10 ml ma tụfuo mgbe gluu ahụ kụrụ nchịkọta.
Ndụmọdụ: pasent nkeAcrrylamideN'ime gluu gluu na protein gluu abụghị otu. Ọ bụrụ na iji emebere Acyrylamide: Bis ngwọta, gbaa mbọ nweta karama kwesịrị ekwesị.
A na-eme ihe nkiri (n, n, n '- Tetrametyl etlelediamine) bụ ihe na-acha aja aja, enịm kerfle. Tinye naanị tupu ịwụnye gluu.
A ga-asacha electrophoresis eji emechi emechi tupu oge ọ bụla. Mgbe electrophoresis, jiri ahịhịa dị nro na-asa mmiri na mmiri ncha ncha, jiri mmiri dechaa ya wee kpọnwụọ.
Àjá na uzuzu nwere ike ime ka polymer nwere ike ime. Tupu electrophoresis, hichaa iko iko ya na iko nhicha ma hichaa ya na ahịhịa dị nro. Jiri mmiri dị larịchaa were kpọọ ya nke ọma. Iri na 70% ethanol tupu izere akwụkwọ na-enyere aka ịsacha ma gbaa ọsọ. Tinye ihe nlere nke acyrylamide nke na-aga nke ọma: BIS, mmiri, nkwapụta ngwọta, amnononium parsulfate, terad. Maa jijiji nke ọma ma wụsa ozugbo.
Ọ dịghị mkpa degass polyacyrylamide tupu polymerization. (Acrylalalide a na-etinye na oghere iji wepu egosipụta n'ihi na oxygen na-egbochi polymerization.)
Usoro nlele ụda ụda.
® tinye mpempe akwụkwọ ojii na igbe nke ala, Black iji mee ụfọdụ oghere iji hụ nke ọma.
Anu ọfụma wee chekwaa ihe ọ bụla.
@ Ma ọ bụrụ na ìhè ahụ nwere ọkụ, gbanye ọkụ, ka ọkụ na-enwu colloid. See ihe nlere ahụ n'ime pipette.
Jikọta ngwa ngwa ngwa ngwa.
Enwere ike iji 10-200 mu 1 mmiri na-agagharị ebe a nwere ike iji ya n'ọtụtụ ihe na nlele ahụ. Maka oghere nlele (na-erughị 10μμ), ndị isi ọkpọkọ ogologo iji maka gluu na-adị mma.
A na-akwanyere ndị na-egbu egbu na ihe nlele ahụ, na-eji nwayọ nwayọ. Nlele ahụ nwere ike ịpụta nke ahụ na glycerin, na mgbapụta ngwa ngwa nwere ike ise ikuku na-egosipụta na isi ọkpọkọ.
Mgbe ị na-eme isi na-abanye na-abanye, ga-agagharị na mmiri na-esi nwayọọ na nsọtụ pipe ma ọ bụ kpochapụ akwụkwọ ịmị mmiri na mpụga nke ụmụ irighiri mmiri. Kpachara anya ka ị ghara ịckụ ihe nlele ahụ.
Debe ihe nlele ahụ n'ime oghere nlele
Ngwaọrụ ngwaọrụ iji mee obere nrụgide, mee ka sachaa ya ngwa ngwa na-ebugharị isi mmiri mmiri.
A na-etinye isi nke pipett na-etinye n'ime nchekwa, dịtụ elu karịa oghere ahụ, na-enwe nrụgide dị mma. M n'ọnụ nke pipette nwere ike itinye n'ime obere oghere.
Na-eji nwayọ nwayọ. A na-etinye ọnụego Pipette n'elu oghere nlele, na ihe nlele ahụ ga-abanye n'ime oghere. Kwe ka nlele ahụ mee ka ọ dị mma iji mejupụta oghere ahụ kama ịkwado.
Ogologo oge ikpeazụ nke ihe nlele, mmiri mmiri ga-aga n'akụkụ nke abụọ, jiri nwayọ bulie mmiri mmiri, pụọ
Etu esi eme ntuli aka glue?
Oghere mpempe akwụkwọ mpịakọta dị n'etiti iberibe abụọ. Na gluu dị mkpa, isi pipette enweghị ike ịbanye n'etiti efere abụọ. Lelee Glycerin! Tinye Pipette isi n'elu oghere nlele na ihe nlele ahụ ga-abanye n'ime oghere.
A na-egbutu ihe nlere anya nke ọma. Sụpụ acyryMolymolide na mmiri nke nwere ike ịpụta na ala nke oghere. Mmiri ahụ nwere ike ime obere oghere. Jiri syri azu ma ọ bụ 50ml ma ọ bụ nke 50ml na agịga 18. Wunye na ebe nchekwa electrophoresis ma jiri nlezianya kpochapu oghere.
O nwere ike isi ike ịhụ oghere nlele, mana n'otu aka ahụ, ndị ọzọ dị mfe. Ọ bụrụ na enwere oghere dị ukwuu, enwere ike ịnwale ihe nlele nwere ike ịnwale ya na bromophenol anụnụ anụnụ.
Oge Post: Mar-31-2023